Utilizing new PlexPlus® technology from SpeeDx
Next Generation PCR Multiplexing
Channel | 52 oC Temperature | 76 oC Temperature |
---|---|---|
1 | Influenza B virus | SARS-CoV-2 |
2 | Rhinovirus/enterovirus | Enterovirus |
3 | Influenza A virus | Human adenovirus (B/C)† |
4‡ | Respiratory syncytial virus (A/B) | Human parainfluenza (1/2/3/4) |
5 | Human metapneumovirus | |
6‡ | Internal control |
Table 1. Viral targets detected with two temperatures per channel.
RespiV PlexPlus® is a 1-well, multiplex qPCR assay that amplifies nucleic acids of the following targets when present: influenza A virus (including H1N1 and H3N2 strains), influenza B virus (Yamagata and Victoria lineages), respiratory syncytial virus (subtypes A and B), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human adenovirus (species B and C)†, human parainfluenza virus (types 1 to 4), human metapneumovirus, rhinovirus (types A, B and C) and enterovirus (types A, B, C and D), shown in table 1.
This reagent includes an extraction/amplification internal control. The reagents are compatible with the Applied Biosystems® QuantStudio™ 5 and 7 real-time PCR systems.
The RespiV PlexPlus® kit is for research use only (RUO) and is not intended for use in diagnostic procedures.
Product | Compatible | PCR Format | Size | Catalogue |
---|---|---|---|---|
RespiV PlexPlus®* | ABI QuantStudio 5 and QuantStudio 7 | 96-well | 100 reactions | 7300201 |
RespiV PlexPlus®* | ABI QuantStudio 5 and QuantStudio 7 | 384-well | 384 reactions | 7300204 |
*The RespiV PlexPlus® components are for research use only, not for use in diagnostic procedures.
†Cross-detection of human adenovirus species A.
‡Custom calibration required. Refer to the relevant QuantStudio platform system manual for calibration instructions.