EntericPlex Bacteria I is a 1-well, multiplex qPCR assay that simultaneously detects and differentiates: Campylobacter spp. (C. jejuni, C. coli, C. upsaliensis, C. concisus, C. fetus, C. lari and C. ureolyticus), Shiga toxin producing Escherichia coli (STEC), Salmonella enterica and Shigella spp. (S. sonnei, S. boydii, S. flexneri and S. dysenteriae)‡. This mix includes an internal control for extraction and amplification. The EntericPlex Bacteria I kit is for research use only (RUO) and is not intended for use in diagnostic procedures.
‡Cross-detection of Enteroinvasive E. coli (EIEC).
Note: ENT-BAC I Mix, 20x provided contains a fluorophore specific for the QuantStudio platforms.
Product | Compatible | Size | Catalogue |
---|---|---|---|
EntericPlex Bacteria I | QuantStudio 5 / QuantStudio 7 | 100 | 7152201 |
*Custom calibration required. Refer to the relevant QuantStudio platform system manual for calibration instructions. Contact [email protected] for assistance.
References
1. Schmidt MA, Groom HC, Rawlings AM, Mattison CP, Salas SB, Burke RM, Hallowell BD, Calderwood LE, Donald J, Balachandran N, Hall AJ. Incidence, Etiology, and Healthcare Utilization for Acute Gastroenteritis in the Community, United States. Emerg Infect Dis. 2022 Nov;28(11):2234-2242. doi: 10.3201/eid2811.220247. PMID: 36285882; PMCID: PMC9622243.
2. https://www.worldometers.info/world-population/us-population/
3. https://www.who.int/news-room/fact-sheets/detail/levels-and-trends-in-child-under-5-mortality-in-2020#:~:text=Globally%2C%20infectious%20diseases%2C%20including%20pneumonia,for%20children%20under%205%20years.
4. Sattar SBA, Singh S. Bacterial Gastroenteritis. [Updated 2023 Aug 8]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK513295/
5. Hewison CJ, Heath CH, Ingram PR. Stool culture. Australian Family Physician vol. 41, no. 10, October 2012
6. MMWR, CDC, April 19, 2013, Vol 62:15
7. Webb, A; Starr, M (April 2005). “Acute gastroenteritis in children”. Australian Family Physician. 34 (4): 227–31. PMID 15861741
8. S. Forsythe, J. Sutherland, A. Varnam. Yersinia, Shigella, Vibrio, Aeromonas, Plesiomonas, Cronobacter, Enterobacter, Klebsiella and Citrobacter. In Woodhead Publishing Series in Food Science, Technology and Nutrition, Foodborne Pathogens (Second Edition), Woodhead Publishing, 2009, Pages 763-801, ISBN 9781845693626, https://doi.org/10.1533/9781845696337.2.763.