Novel nucleic acid amplification approach, creating amplicons distinctly different from the parent sequence. The combination of PlexPrime® and PlexZyme® technology enables multiplex mutation detection with high sensitivity and specificity.
The PlexPrime® primer is composed of three regions:
The unique feature of the PlexPrime® primer make it extremely suitable for detection and discrimination of mutations or Single Nucleotide Polymorphisms (SNPs) in a multiplex reaction or a pool of nucleic acid sequences containing non-conserved regions.
Tan L. Y., Walker S. M., Lonergan T., Lima N. E., Todd A. V. Mokany E. 2017. Superior Multiplexing Capacity of PlexPrimers Enables Sensitive and Specific Detection of SNPs and Cluster Mutations in qPCR. PLOS One January 23rd.