High performance and reliable multiplex qPCR detection. PlexZyme® is the driver behind the entire SpeeDx product portfolio.
PlexZyme® technology offers high performance and reliable qPCR detection and is the driver behind the entire SpeeDx product portfolio. During PlexZyme® qPCR, primers amplify target nucleic acid sequences and produce amplicons which serve as a template for PlexZyme® formation. Once the partzymes have assembled into PlexZyme® enzymes, universal probes bind and enzymatic cleavage of the probes between fluorophore and quencher dye pairs generates fluorescence. Changes in fluorescence allow detection and/or quantification of the target nucleic acid in real time.
Novel nucleic acid amplification approach, creating amplicons distinctly different from the parent sequence. The combination of PlexPrime® and PlexZyme® technology enables multiplex mutation detection with high sensitivity and specificity.
The PlexPrime® primer is composed of three regions:
The unique feature of the PlexPrime® primer make it extremely suitable for detection and discrimination of mutations or Single Nucleotide Polymorphisms (SNPs) in a multiplex reaction or a pool of nucleic acid sequences containing non-conserved regions.
Mokany E., Tan Y.L., Bone S.M., Fuery C., Todd A.V., 2013. MNAzyme qPCR with superior multiplexing capacity. Clin. Chem. 59(2); 419-426
Mokany E., Bone S.M., Young P.E., Doan T.M., Todd A.V., 2010. MNAzymes are a versatile new class of nucleic acid enzymes which can function as biosensors and molecular switches. J. Am. Chem. Soc. 132(3); 1051-1059
Tan L. Y., Walker S. M., Lonergan T., Lima N. E., Todd A. V. Mokany E. 2017. Superior Multiplexing Capacity of PlexPrimers Enables Sensitive and Specific Detection of SNPs and Cluster Mutations in qPCR. PLOS One January 23rd.